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Let Your True Colours Shine Through - Chromatography

Here's two of our experiments - to find out the results keep reading....

You WILL! need

green leaves - we used spinach

black pens - one water soluble, one permanent

blue-black ink

containers ( we've got flasks, but you could use jam jars)

pipettes

:p ( don't include this! ) I SAID DON'T!!!!

scissors

mortar and pestel

rubbing alcohol

filter paper - you could use coffee filters, but they need to be white so you can see the colours

clips

pencil

ruler

water

Our aim was to investigate what colours (or pigments) are really in leaves and different kinds of ink (!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!)

oh wrong photo sorry......

LETS ZOOM INTO THE FUTURE!

Cut up the leaves in the mortar, add a small amount of alcohol and prepare to MASH!

mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V. mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V mashy mc mash face! :V

add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :Ov add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O add rubbing alchohole :O

MASH & BASH!!!!!! MASH & BASH!!!!!! MASH & BASH!!!!!! MASH & BASH!!!!!! MASH & BASH!!!!!! MASH & BASH!!!!!! MASH & BASH!!!!!!

Keep going until the alcohol goes bright green.

MEASURE!!!( THE HEIGHT AND WIDTH OF THE CONTAINERS YOU ARE USING )

CUT FILTER PAPER SO THEY ARE NARROWER AND SLIGHTLY TALLER THAN THE CONTAINERS

FOR ONE WE DID 2CM BY 4CM ON FILTER PAPER

DRAW A 1CM LINE UP FROM THE BOTTOM AND PUT A LINE IN THE MIDDLE - THIS IS WHERE YOU WILL LOAD YOUR SAMPLES

USING A PIPETTE, ADD A DOT OF ONE OF THE SAMPLES ( PENS, INK OR GREEN LIQUID FROM THE LEAVES)

PUT A SMALL AMOUNT OF SOLVENT INTO THE CONTAINER (ALCOHOL FOR LEAVES AND PERMANENT PEN, WATER FOR ORDINARY PENS AND INK)

SUSPEND THE FILTER PAPER SO IT JUST DIPS INTO THE SOLVENT

SEE THE SOLVENT ZOOM UP THE FILTER PAPER

AND........

NOW THE RESULTS....TAH DAH

SEE ALL THE DIFFERENT COLOURS OR PIGMENTS

BLACK MARKER PENS (NORMAL ON LEFT, PERMANENT ON RIGHT)

NORMAL BLACK INK IS NOT BLACK AT ALL - IT'S LOTS OF DIFFERENT COLOURS MIXED TOGETHER including blue, red and yellow

PERMANENT BLACK INK DOESN'T SEEM TO BE MADE UP OF DIFFERENT COLOURS

LEAVES - THE SAMPLE HAS SEPARATED INTO AT LEAST 4 DIFFERENT BANDS OF COLOURS. THESE ARE THE PLANTS PIGMENTS. THE TWO GREEN ONES ARE DIFFERENT KINDS OF CHLOROPHYLL - THE CHEMICAL WHICH ALLOWS PLANTS TO PHOTOSYNTHESISE. THEY USE THIS TO CAPTURE ENERGY FROM THE SUN TO GROW. THIS IS THE START OF EVERY FOOD CHAIN ON EARTH. GO PLANTS.

BLUE BLACK INK - LOTS OF DIFFERENT COLOURS, SO IT'S MADE UP OF DIFFERENT INKS

THANK YOU AND BAI!

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